-
While trying to run superfreq on a set of 8 samples the program stops after an hour or so running saying that there are no reads counted in any of the samples I have. I have checked the files and they…
-
During live discussion with @CuriousGeorgiy some interesting conclusions have been drawn. Let's dive into memtx MVCC origins. Why do we need it at all?
## Dirty reads
The primary problem of memtx wi…
-
Given that FASTQC takes a large chunk of time, Mike suggested running FASTQC on a subset of reads. Will agrees that this would be good to do, we would just need to calculate the total number of reads …
-
Hello!
Thank you for the tool and all the support!
I'm trying to compare an ONT 16S sequencing with V4 Illumina using EMU and different databases. I was wondering about subsampling and how this …
-
Dear all,
fantastic software, many thanks!
I have a very naive question. After simulating reads for AmpliconSeq data, I would like to basecall the reads with guppy and then use these FASTQ rea…
-
I am trying to create a consensus sequence (or consensus sequences for a few of the most abundant morphs) for the rDNA repeats of several mammal species that do not have existing rDNA reference sequen…
-
For drgn scripts running against the live kernel, time spent in the kernel reading from `/proc/kcore` is often the bottleneck (apart from the syscall overhead itself, which we can't really avoid). The…
-
hi
short reads from parents are required in Trio binning processing. but I have long reads (pacbio) for parents and child, can I still go through the same processing for the child?
thanks
-
Hi,
I'm getting this error when using extract_kraken_reads.py, could you help me solve this error?
```
python /home/ga-fr-noy-linux02/KrakenTools-1.2/extract_kraken_reads.py -s1 cseqs_1.fq -s2 cseq…
fetyj updated
3 hours ago
-
Hello,
We are doing high-throughput cloning of different gene variants and want to move the QC of our cloning products to Nanopore sequences. I did a trial run with a library of ~400 variants. The …