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Hi, Felix.
I'm so sorry to bother you again. I have WGBS data from the same batch for two species (Accel Swift data) and have already trimmed it using the method you suggested, with the following c…
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Here is my procedure. First I ran dorado basecaller, where I required all modifications to be called:
` dorado basecaller -v sup,inosine_m6A,pseU,m5C --min-qscore 9 --emit-moves -b 64 --mm2-opts {p…
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Hi,
Thanks for developing a convenient tool for the biologist, like me, to quickly establish a local analysis platform. However, there are only Human and Mouse reference genomes in the database. I…
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Wir sollten uns auf ein einheitliches Genom einigen, durchprobieren können wir evtl mehr. Wie Lydia meinte, Ensembl GRCh37 ist ~30 GB, NCBI build37.1 ~15 GB, ist also nicht ganz so einfach. -Christoph…
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Hello,
When deciding which SGBs were similar to reference genomes in public databases, what distance and p-values cutoffs were used?
Cheers,
-Nelson
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I'm curious if this process works when one maps the reads to a reference genome for another species. For example, we have low-coverage reads (``14x) for one species and have inferred a reference-based…
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I am trying to merge gVCF files of UKBIOBANK to eazify the annotation process, but the merging tools in GATK, or BCFtools have a problem with the reference. Although UKBioBank states GRCH38 is used ,…
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Hello, When I used the GCI software to assemble my T2T genome, the following error occurred. What happened?
Used arguments:{'reference': '/export/home/zzl/YQ/Genome/Mel.genome.fa', 'hifi': ['/exp…
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HI
I am using the blender on a WGS for two BAM files, I am getting this error despite all the reference genome files that I have used
$ sudo sh run_blender.sh hg19.fa.align /datadrive/info.j…
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**Describe the bug**
I am trying it for the first time. Can you tell me why loading a reference genome takes so long?
**BISCUIT version**
Program: BISCUIT (BISulfite-seq CUI Toolkit)
Version: 1…