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Hi all,
I'm using the barcoding kit SQK-NBD114.24 for sequencing on MinIon Flow cell. Before the library preparation and sequencing, I'm doing on each samples a pcr with barcoded primers:
![image]…
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Dear All,
Can I use Mykrobe tool for targeted sequencing instead of whole genome sequencing?
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### Checked for duplicates
Yes - I've already checked
### Is this a regression?
No - This is a new bug
### Version
1.6.1
### Describe the bug
When creating a moderate-sized expans…
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**User story**
As a developer (Ben) I would like there to be fine-grained batch creation validation for sequencing pipelines.
Currently batch validation for sequencing pipelines lives in a few pla…
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We routinely use Minimap2 for processing large nanopore sequencing datasets which take hours. For example, aligning a single 30x dataset to the human genome using Minimap2 takes approximately 12 hours…
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```
PATRON: You may then hire a client from the top of the deck.
```
The "then" makes it sound like this is in addition, rather than adding an option for where to take from
epage updated
5 months ago
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Hello,
Can the same pipeline be used for cDNA sequencing as well?
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**Is your feature request related to a problem? Please describe.**
Agenda section which would allow agenda items to be grouped together. The section will require:
A description
An ordered sequence …
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Hello, thanks for your great approach!
In the github project, we found that you only provided mouse aging_gene_sets. Can I use these gene sets for human single-cell sequencing? If not, can you provid…
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Hi @alexdobin,
Apologies: this question has already been asked a few times, but the last time you answered was in 2018 I think and I'm just wondering whether anything's changed since then.
- Doe…