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Hi,
I seem to have the same problem as described in the closed issue #244 using --nextseq-trim on paired-end data. Poly-G tails in reverse reads were not removed although I specified the --nextseq-t…
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Hello,
I am writing to you because I am having trouble understanding the output of my results. I have performed a RNA sequencing with RNA004 and I want to know which sites are m6A. When I get the t…
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# Issue Report
## Please describe the issue:
Hi, I was testing different configurations to demux non-ONT barcodes in order to find optimal scoring and window sizes settings, and found inconsiste…
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Thanks for this amazing package! I have used it in so many projects already!
I just wanted to check if there are plans to support sparse matrices as well any time soon?
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Hello again! This is a follow-up to issue #247, thank you so much for your insights there!
So I managed to run TRUST4 on my SMARTer data with the following command (which still took really too long…
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Configuration file: db_main.toml
Description: miRDB is an online database for miRNA target prediction and functional annotations.
```toml
[db_mirdb]
source_url = "http://mirdb.org/download/miRDB…
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Can this pipeline also demultiplex reads from cell barcodes?
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Hi,
In both single-cell and bulk RNA sequencing, we use UMIs (Unique Molecular Identifiers). Two reads with the same UMI correspond to the same cDNA molecule. It would be great if we could feed inf…
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Hi,
Can you please give a suggestion on how to use SEACR with experiments that have spike-in and replicates. What do you recommend as far as normalization with spike-in and how to handle replicates?
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