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Might it be advisable to use NR or NT databases to count full-length Trinity transcripts instead of SwissProt?
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**Operating system**
`Centos7`
**Package name**
`bam2fasta`
**Conda environment**
```
WARNING conda.core.prefix_data:_load_site_packages(272): Problem reading non-conda package record at lib…
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1. abricate
2. bali-phy
3. bandage
4. berokka
5. bonsai
6. cannoli
7. circos
8. consel
9. elph
10. ema
11. emboss
12. exabayes
13. fastp
14. finch-rs
15. flye
16. genome-painter
17. gf…
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#### I'm trying to do following to assemble a diatom:
(1) Kneaddata/Trimmomatic for my transcript reads;
(2) Mapping the reads to my unmasked genomes;
(3) Using RNA-Spades for the transcript as…
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I've contacted the developer of NGLess with this question, but it has been about a week and he hasn't responded so I'm hoping someone here can help!
I have managed to successfully assemble metagenome…
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I'm having the issue mentioned above. I feel like I must be missing something obvious, but I can't see it.
Any help would be greatly appreciated.
INPUT:
```
/home/shared/TransDecoder-v5.1.0/T…
kubu4 updated
6 years ago
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Hi all,
I installed the latest version of FALCON with your instructions, on my CENTOS server. I created a new conda environment and I used this conda to install FALCON with the command : _conda ins…
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Hi,
I tired to use the gtf_genome_to_cdna_fasta.pl, but got the following error:
Can't locate Gene_obj.pm in @INC (@INC contains: /mnt/home/lw/Software/../PerlLib /opt/moab/lib/perl5 /usr/local…
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i have 6 sample of RNA seq data . i run trinity as a result i have 6 trinity.fasta files. Now i have to find functional annotation , for that i have to use trinotate software. can i merge all these tr…
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Dear FEELnc,
During my running I've got an errors.
It generated same error in Shuffle and intergenic mode.
Do you have any idea?
Thanks!
FEELnc_codpot.pl -i candidate_lncRNA.gtf -a ../../…