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Hello.
I am comparing 16S rRNA primers to understand their impact on the community composition of my samples.
The two primers are 515F_806R and 341F_805R.
Amplicon libraries were prepared …
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Hello,
Thank you for coding this nice software. I was wondering whether there is any recommendation or previous tests that indicate an optimal or recommended threshold to work with mumu for 16S amp…
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Hello!
Thank you for the tool. I run it with some samples, and the results are promising.
Currently, I am testing this tool for one pipeline that should handle many ONT barcoded samples.
I have alr…
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Hi,
Now, I collected 9 datasets about 16S amplicons from human gut microbiota, and the datasets were be merged. I need to test the taxon difference between two groups (Ulcerative Colitis cohorts vs…
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working on a server with Ubuntu 22.04.4 LTS
conda 24.1.2 installed
I followed the instructions on https://docs.qiime2.org/2024.2/install/native/ and chose linux amplicon
* wget https://data.qiim…
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Thank you for this great tool! We have been doing viral phylogenetics and this has been great. I wanted to gain a deeper understanding re: --allreads and default parameters. We've had more consensi se…
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I am receiving a "segmentation fault" error when I try to run DEICODE auto-rpca. I've tried running it both in QIIME2 and standalone, with the standalone as the most recent version installed with con…
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Hi @benjjneb
I am currently working on some ITS2 datasets that have been generated separately from different substrates, and therefore differ slightly in the library preparation. The goal is to ru…
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Hello!
I am having difficulties to explain the expected and the true final amplicon size (after preprocessing). I am reading some forum posts in which @benjjneb suggests that v3v4 amplicon size sho…
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In my collaborations, I often encounter situations where PhD candidates are volunteered by their PI's to also handle amplicon analysis, but are total microbiome newbies. Situations might be complicate…