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**UniProt UniRule (ID and label):**
GO:0032968 | positive regulation of transcription elongation from RNA polymerase II promoter | IEA with UR00063877
**Sequences with problematic annotation (ID…
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**1. What were you trying to do?**
I used `vg rna` to build a spliced pangenome and a pantranscriptome with a GFA file from PGGB and a gff3 file. Similarly, I used `vg autoindex` to build index f…
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Dear Greg - thanks for your continued work on this and for this follow up paper with updated pipeline. I'm the lead author of [Hermida et al. Nat Commun 2022](https://www.nature.com/articles/s41467-02…
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Hello Team,
I have a basic query regarding the fastq reads , i don't have fastq reads in pairs, i have single fastq reads generated from direct rna sequencing(SQK-RNA002), is it possible to run DIC…
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Hello!
I have cDNA and direct RNA Nanopore reads which contain poly-A/poly-T sequences and adapters/barcodes.
A friend suggested me to use pychopper to remove those poly-A/poly-T sequences and a…
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Hi,
I want to plot kmer values of direct RNA sequenced samples, and got it to work successfully, but it appears that C and U center bases have the lowest signal, while A and G center nucleotides h…
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Opening a ticket for discussion regarding the terms:
**transcription assay evidence - ECO:0000168:**
A type of direct assay evidence resulting from the detection and quantification of transcribed …
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Hi Sam, its me again here.
Tried running the program again with TCGA samples, but unfortunately immediately ran into this new error.
When running the preprocessing, I realise that the tag direc…
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Hi, I hava two problem in learning nanocompore.
1. The following files are provided in https://www.yeastgenome.org/strain/SK1 about reference file site mentioned in paper. Is the `SK1_NCSL00000000_S…
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**What is the SO term name and accession?**
gRNA_gene (SO:0001264)
**Describe what you would like to change.**
Current def is "A noncoding RNA that guides the insertion or deletion of uridine res…
sjm41 updated
2 years ago