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Hi,
I've got some fastq files from a human DropSeq experiment with single nuclei, and I'm trying to put together an index using a sequence from an mRNAi resistance gene. The idea is to check if we…
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https://mp.weixin.qq.com/s/E1LKT0LYd2K_6vfOhFAnQA
ixxmu updated
2 months ago
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https://mp.weixin.qq.com/s/4JmnEOvb_d8TVdWpXDDizg
ixxmu updated
4 months ago
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Hi @mlist,
I opened this issue to coordinate the next steps in our benchmarking study.
I'd say the first important decision to take regards the **single-cell data** we use (for signature buildin…
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### Instructions
Hi,I'm trying to run DigitalExpression step but I get the empty matrix.I have already done the step as cookbook.Such Tag the barcode,UMI,merge bam file and tag exon.These no any erro…
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Congratulations for the great code. I just run in a possible bug of the current (0.7.5) version of `spacemake projects add_sample` command.
I have generated the projects_df.csv using the `spacema…
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get assay nodes from FlyBase - with methods (10x or dropseq) annotation
see example http://flybase.org/reports/FBlc0005438
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When processing the data in Scanpy I am unable to figure out why my plot of the Highest Expressed Genes shows up with numbers rather than gene names as the identifiers on the Y-axis.
Example:
…
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Hi，
Thank you for your contribution to picard tools.But when I try to use Picard FastqToSam the following error has occurred and I wonder how to solve this problem.
```
picard.sam.FastqToSam done.…
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Starting with a fresh installation (v0.7.2), adding the `--download_species` during initialization
`spacemake init --download_species human --dropseq_tools /Drop-seq_tools-2.5.1/`
throws an erro…