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Hello,
I am currently having an oligo designed with m6A modifications to serve as a control strand for epinano SVM. The oligo will have an RRACA motif, where the first adenine is m6A and the second…
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Hi again,
I was wondering how the upset plot data is laid out for individual assignment. It looks like you converted singlets to "1" and anything labelled as a doublet to "0" for the upset. However, …
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Hi,
I believe the following dictionary keys are output by 1.2.2 but not 2.0.0:
PRIMER_PAIR_0_PRODUCT_TM_OLIGO_TM_DIFF
PRIMER_PAIR_0_T_OPT_A
PRIMER_LEFT_0_POSITION_PENALTY
PRIMER_RIGHT_0_POSIT…
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Hi,
I am having issues running the practice gene tutorial. 0 SNPS found.
Please see output below.
$ mipgen -regions_to_scan practice_genes.bed -project_name practice_design -min_capture_size …
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Hi,
I have run the mipgen_practice with the example commands but I don't get any logistic scores, only -nan values. I have read the previous posts regarding log scores /nan values, but the error mess…
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https://mp.weixin.qq.com/s/BlGfUheeUjkLKDVjlIY4XA
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I took the example from the documentation for a `design_primers` run, and tried to add a `Dict[str,str]` for the mis-priming library (`misprime_lib`). I get an OS error: failed to allocate memory, alt…
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Hi,
Would it be possible to generate mips against a known sequence without having to run it against the reference genome and skipping the BWA part? I would like to use RNA isoforms and short oligos…
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Hello,
Finally I let the MIPgen software run properly and get my probes designed.
But I am still not quite sure about several things. Therefore would like to ask for advice or suggestions.
1. Every tw…
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Hello,
I am in the process of designing a smmip panel on secific variants (snvs/indels). As I am trying to capture point mutations, the size of the captured region is not very important and does not …