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貼吧活動：(請查閱 [SARS-CoV-2 Timeline by 2020.02.21](https://github.com/agorahub/_meta/blob/agoran/theagora/sari/Memorandum_2020-02-21_SARS-CoV-2-Timeline_Nathan.pdf?raw=true), by Nathan :cloud: )
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Dear Brian,
I accidentally analysed i.e., assembly and mapping, stranded RNAseq data (dUTP protocol) as unstranded. After noticing, I reanalysed the data set in strand-specific mode. When comparing…
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Hi,
I have a question about the script that does the translation of ORFs to genome coordinates. There are a fair number of sequences that have the error 'shouldn't have a minus-strand ORF on a spli…
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I'm trying to create a new network, I wrote the .json file. In Python I write:
- config = from_json("CPG_bsb4.json") - OK
- scaffold = Scaffold(config) …
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Hi,
I do not plan to use GeneMark-ET but I want to use Augustus, GlimmerHMM and SNAP.
1. If I ran `funannotate train` do I still have to use `funannotate predict --rna_bam `? If yes then what is …
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Hi,
We are trying to generate .tbl file for NCBI submission using convert_gff3_to_ncbi_tbl.py script at biocode. But the problem is, GFF3 produced by Transdecoder has multiple genes predicted for s…
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Hi,
I performed transdecoder on 13 RNA samples at first, and then included 18 more samples to perform gene prediction with transdecoder. Using the same genome( repeats unmasked), I was able to pred…
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HI,
> If you have RNA-Seq data and have reconstructed transcripts using a method such as [Cufflinks](http://cole-trapnell-lab.github.io/cufflinks/) or [PASA](http://pasapipeline.github.io/), you ca…
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Hello,
Thank you so much for publishing this method! I'm excited to try it out on our data and have two questions.
First, how do you recommend handling missing data? Specifically, we work with l…
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I had this idea :
Youtube video subtitles are pure written form of human spoken words plus there are so many great videos out there , courses and content that is very precise in teaching and solvin…