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Add ![image](https://user-images.githubusercontent.com/1882209/225792910-95aef49d-4ef6-405a-89ab-636293636168.png) [here](https://github.com/CCBR/CARLISLE/blob/61249518bf7fd63603e2230d8dd3d098c34825d7…
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I would like to retrieve the alignment information for a given secondary or supplementary alignment .
For example, in duplicate marking, we may choose to use the primary alignments of template (rea…
nh13 updated
4 months ago
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Hi remora team,
I tried to run remora dataset prepare for my reads sequenced in r9.5 chemistry, but got error as shown below.
![image](https://github.com/nanoporetech/remora/assets/76148123/0cdf4fbc…
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Hello,
**Question 1.**
I prepared bam file from pod5 file.
This is how I prepared bam file.
[hjk@PC24B090:/data_ngs3/hjk/data/nanopore_dorado_test]$ `dorado basecaller /data_ngs3/hjk/utils/d…
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Hello, I am having an issue where I convert paired fastq's to ubam, then run the assemble_refbased pipeline, but 0 reads align despite no errors coming up in either of the steps. These are fastq's sim…
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I met the error when installing the SPAdes.
Log is here
[log.txt](https://github.com/Xingyu-Liao/LongRepMarker_v2.0/files/8650904/log.txt)
.
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After running `bwa mem`, `samtools view` and `samtools sort` to obtain a suitable bam file for `samtools mpileup`, and verifying each output with `Picard ValidateSamFile`, what i get is an empty mpile…
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Hi,
I tried to run f5c eventalign using the merged blow5 file that I created from slow5tools and here is the error I got:
```
[slow5_idx_init::INFO] Index file not found. Creating an index at '…
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Hi everyone! I'm using Dorado for the first time to identify the presence of methylated bases on mitochondrial DNA.
After having installed and correctly used the modkit tool to create the bedMethyl f…
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Hey there,
First of all, congratulation on the exciting new release!
What is the expected runtime for `dorado correct`? I've got around 2 terabases worth of ultra long sequencing and I'm wonderi…