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I am sequencing barcoded samples on a MinION and performing basecalling and demultiplexing using dorado server in MinKNOW, with barcode trimming and mid-read barcode filtering enabled. Approximately 2…
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Hi,
Small question regarding the automatic model selection with dorado.
I ran sequencing at 260 bases per second but dorado appears to select 400bps for basecalling.
Fyi, the POD5 (using ins…
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# Issue Report
## Please describe the issue:
I compiled Dorado from source and it finished without errors. I then proceed to tests and they both fail.
sampajanna:~/Downloads/dorado$ ctest --t…
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When basecalling with Dorado 0.6.1 it is not successfully assigning barcode groups/demultiplexing using kit SQK-RBK114-24.
We have tested basecalling the same small POD5 file and have varying res…
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I want to use dorado to basecall data of rna001 kit, but models for rna001 kit was not provided. Do models for rna002 kit support data of rna001 kit? Thanks you
ssscj updated
3 months ago
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### Basic questions:
- [X] I am actually using ketting, and not any other software (e.g. magma)
- [X] I am using the latest Ketting version at the time of writing for the Minecraft version, that I…
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Hi all,
My HPC uses multi-instance GPU (MIG). Despite booking several MIG with slurm (`--gres=gpu:3`) it seems Dorado uses only 1. I have tried running dorado with `--device cuda:0,1,2` but I get t…
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# Issue Report
## Please describe the issue:
When I want to download a model for basecalling, `dorado download ` gets all models that can work with my pod5 data file.
Using `--model sup` can …
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Hi,
I would like to ask a question about using Dorado for basecalling sequencing data obrained with the RNA004 kit:
when looking at dorado basecaller -h I cannot find the --moves_out option. Howve…
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Hi, I have successfully run Dorado with RNA004 m6A model and used modkit to convert my modbam file to bedmethyl output.
`${DoradoDir}/dorado basecaller --min-qscore 7 --verbose --emit-moves -k 14 --s…