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Hello,
I have been mapping Rhapsody WTA libraries using STARsolo with great success using the suggestions [here](https://github.com/alexdobin/STAR/issues/1111#issuecomment-1059029529) however, BD jus…
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Hi Dr. Zhu,
While working on the provided data in original paper(GSE152020), I realized that there are a few different ways to group samples, including sublibraries, replicates(rep1, rep2, and rep3…
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Thank you very much for providing so much data and code in such an organized fashion.
I am interested in the LSI projections and reclassification of the AML cells as in fig 7.
Perhaps it should be i…
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Hi,
Thanks for developing this suite of tools! I am using umi-tools for a custom application where multiple barcodes are attached to the read name. My understanding is that the last field of the re…
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> res = census_main(pbmc, organ = 'Lung',verbose = T,predict_cancer = T)
Running Seurat
Warning in PrepDR5(object = object, features = features, layer = layer, :
The following features were not…
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With programs like Seurat and Scanpy + scirpy, we visualize the gene expression and VDJ data. I am wondering what output files from Trust4 can be used for the same purpose. Do we need to create the a …
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Project UUID: c4077b3c-5c98-4d26-a614-246d12c2e5d7
Project Title: Ischaemic sensitivity of human tissue by single cell RNA seq
Project Short Name: TissueStability
Submission UUID: fd52efcc-6…
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Hi,Thanks for developing such a good tool!
When I am using the getbarcodes step ,I use the following command:
GetBarcodes(fqFolder = fastqFolder, BCClustAssignFile = bc_clust, outputFolder="/data/R0…
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Good morning,
I've a problem converting an HTML page with barcode into pdf with the latest wkhtmltopdf version: the barcode is a code128 barcode and when printed from HTML, scans fine; when we run i…
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Hi,
Wondering if there is a guideline setting the UMI and promoter ration thresholds for barcodes selection? Thanks!!