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### Ask away!
Under the advanced basecalling options, what arguments are accepted for demux_args? It is described as `Additional command line arguments to pass to the basecaller barcoding process.` b…
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Hi,
I am using the PolyA tail estimation in plasmids and I get shorter lengths than expected. I have a few questions about how to set up this feature.
The plasmid I sequenced has a very long poly…
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Hello,
I want to detect 6mA, 5mC, and 5hmC for R10 DNA with Dorado, so I need to use three models , using the Automatic model selection complex mentioned in the documentation.
However, I found …
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Thanks so much for making such a useful tool @hasindu2008 et al. - it's been a delight to work with!
I unfortunately needed to do a Blow5 to Pod5 (...) conversion with `blue-crab` ( :heart: @Psy-Fe…
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Hello,
I am trying to extract methylation data from a modbam file basecalled with Dorado using the RNA004 kit. I have sorted and indexed the file with samtools, but when I run modkit pileup, it out…
esh97 updated
3 months ago
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Hello, we are running dorado on 2 samples. One native DNA vs the PCR amplified one that "in theory" should not hold the methylation.
Why do we get a methylation signal in the PCR sample?
The methyl…
BioRB updated
1 month ago
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Is the dorado correct with "-m herro-v1 " can replace Herro (model_v0.1.pt) for R10.4.1 data?
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Hi! I am pretty new to Nanopore sequencing/bioinformatics in general so apologies if I am missing something obvious here. I used dorado base calling to generate BAM files with tags for modified bases …
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* link to support ticket: [#2024052260000364](https://otrsdict.ugent.be/otrs/index.pl?Action=AgentTicketZoom;TicketID=157170) + [#2024052260000801](https://otrsdict.ugent.be/otrs/index.pl?Action=Agent…
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## Demuxing with a sample sheet produces two bam files per barcode
I use a sample sheet with the `demux` command with aliases, and it outputs both a bam named with the alias and a separate bam with…