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Hi I have a simple question about using `dorado trim`
I did an amplicon sequencing run using the EXP-PBC096. The sequencing was run with live base calling using MinKNOW. However, the HTML report sh…
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I have a bam file containing simplex and duplex reads, I need an output file with duplex only reads, I used the following commands to do so, but once an output file is generated, it seems to be empty
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Hello,
I have few question related to the compatibility between Clair3 model and Dorado model.
_ I would like to know what could be the differences on my variant calling if I switch Clair3's mod…
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Hello,
I am using modkit pileup to create a bedmethyl file for modified basecalling.
I basecalled with Dorado, afterwards I sorted the bam file.
However when using modkit, i get an empty bed file…
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# Issue Report
A subset of my pod5 files of a specific run take 6x longer to basecall than normally / the other files. This was a promethION run and didn't encounter on another run so far.
## De…
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Hello,
We created a nucleic acid molecule structure like "3' ---- ADAPTER ----DNAadapter---- poly(A) ---- RNA ---- 5' "，and we need to accurately estimate the length of polyA. What changes doe…
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Hi, I am using DNAscent 4.0.2 from singularity, and MinKNOW 23.04.5 and Guppy 6.5.7, generated .pod5 data from PromethION 24 using R10 flowcells. I have converted pod5 to fast5. Because I am using an …
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I have used dorado base called the data without --min-qscore . I am now trying to filter the bam file (with base modifications) based on q score. Is there a way to do that, or I have to re-base call i…
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Hello dorado developers,
I know that for software like Guppy putting a direct link to a Guppy download was not allowed as it was proprietary.
But given Dorado is open-source, it shouldn't be a …
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Forego one-size-fits-all Map & Legend Binning and give developers/users the ability to define Map & Legend Bin-size themselves for as many Bins as desired. For SQL based Questions it is doable.
I…