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Hello,
I'm currently trying to use dada2 to analyze some pacbio hifi amplicon data that is for near full rRNA operons (~ 4kb). I have a few questions about the optimal way to run dada2 for my data…
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I did ITS sequencing of stool samples for Fungi. However when I look at relative abundances, even for phylum 60-70% of my relative abundance graph is Other DNA.
When I look at how my ASVs map with…
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Establishment of a non-Westernized gut microbiota in men who have sex with men is associated with sexual practices – Kun Huang – Cell Reports Medicine
https://www.cell.com/cell-reports-medicine/ful…
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# After VALIDATE:
![image](https://github.com/bio-tools/biotoolsRegistry/assets/992660/fca6643c-2b68-4699-ad0f-84210c29f4fa)
# Other weird things:
- What is the red rectangle around SAVE??
- VAL…
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I will continue the discussion here, until or if @keean can get Github to restore the thread. Luckily I still have a copy of the deleted #35 thread loaded, so I can copy and paste from it. If the orig…
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Hello Ben,
I have two questions about my latest sequencing project.
Firstly, I would like to compare the relative abundance from two different studies - one study is the American Gut Project, wh…
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I'm working on a lab for radEmu (relevant lines below), and noticed something suboptimal about printining `ch_fit` vs `ch_fit$coef %>% head`
The column `covariate` in `ch_fit$coef %>% head` lists `…
adw96 updated
5 months ago
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I'm trying to analyze a set of Paired FastQ files with dada2 in R. In my analysis I was doing before I realized that the raw reads still had their primers attached. So I went through the ITS workflow …
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Hi there,
Thanks for developing this amazing tool! I tried to apply it to paired-end single-cell sequencing reads, following the tutorial with the commands below
```
Rscript /data3/zhangdw/01.T…
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## Initial Request
### Goal
_Describe what you're trying to accomplish. This is the only necessary step to start this process. The Committee is available to assist with all other steps. Please cle…