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Hi, I am running into memory problems with a task that should be easily doable (I think). I'm running 1400 samples from a 100 amplicon sequencing experiment through freebayes for variant calling again…
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I am getting stuck at the chimera removal step when analyzing my Illumina 16S data, sequenced in a asymmetric 100x400 configuration (as detailed [here](https://microbiomejournal.biomedcentral.com/arti…
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Hi,
First of all thank you for this algorithm which I hope will solve the problem of ITDs in NGS.
I'm doing a comparative study between the different algorithms in this problem. Currently, I can…
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Do we need to have the ability to download data, run the pipeline, and upload the results?
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It'd be nice if host subtraction was optional and not performed by default on certain samples.
We typically perform background sequence subtraction using Kraken2/Centrifuge taxonomic classificatio…
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Hi everyone,
If you have any issues with the code, please post them here. We will get back to you as soon as we can. Thanks!
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As I explain to other researchers the merits of DADA2, I am always at a loss as to what to call the final sequences from the DADA2 results. I usually just say "OTU", because it is easy to understand, …
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Hi,
I downloaded ONTBarcoder_0.1.8_exe.win-amd64.zip, unzipped and started the executable ONTbarcoder. The GUI starts and I am able to select input files. However, just after starting the analysis, t…
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HI!
Thank you for great work with the tool. I'm evaluating it for our work in extraction molecular barcodes from NGS amplicon sequencing. In general it looks very promising, but I have one strange …
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Dear Ben,
Firstly I would like to thank you for all your amazing work. I really appreciate it.
You might be able to help with an issue I have found with my samples. I have 16S amplicons obtained wit…