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Dear author,
Happy Thanksgiving! Thank you for presenting such a nice software, we are wondering if we could run the pipeline without docker. Could you please give us some hints about how to deplo…
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Hi Simone,
Thanks for sharing this work.
I'm using MetONTIIME to process ONT data after 16S barcoding protocol.
For certain genera and species, I'm getting over 10k features, it was a lot to proc…
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Hi,
I have a read pattern which is something like that:
1) the first cell BC part (16bp among a collection of 96)
2) a fixed known sequence of 4bp
3) the second cell BC part (16bp among a …
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Update the following URL to point to the GitHub repository of
the package you wish to submit to _Bioconductor_
- Repository: https://github.com/PengfanZhang/Rbec
Confirm the following by editin…
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I don't know exactly how to do this, but, as a start:
after linearization all amplicons end up having the ORF split into `sequenced_mRNA_1` and `sequenced_mRNA_2`, so maybe I could just make a table …
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I am currently using DADA2 to analyze amplicon sequencing data. There is a special demand for my project to find out which ASV each read is assigned/corrected to. I am wondering does DADA2 support the…
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I've attached my code and output log file below, would highly appreciate any guidance I can get on this please!
Output log file:
INFO [2020-10-19 17:22:49] Loading PureCN 1.18.1...
INFO [2020-10-…
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I have a usecase in mind were rather than adapter or primer sequence which I want to match and remove, I have markers for a region of interest, and I want the (possibly inexactly matched) marker to be…
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Hej!
Thanks a lot for developing this wonderful tool! Before this tool, we just trim our reads by experiences of looking at the sequencing report, which is not very "scientific".
But I am confused…
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Dear all,
I am wondering if I might loose too many reads after filter and trim. I am using V3V4 16S primers and my expected amplicon size without primers is 427. I used cutadapt to remove my primer…