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Hi,
thanks a lot for this software. The concept is very convincing to me.
I just like to ask, why the base quality score is not used during the clustering and de-replication process?
Is it not poss…
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Is there a way to change the stacking order in a plot_bar graph (not the legend order but the stack order)? As it is now, each stack is ordered with the factor composing the largest abundance on the b…
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Tried with v 0.6.0 and 0.6.1. Memory allocated 20GB, used
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Hi,
I am analyzing 16 microbiome data from the lung and mouth and I'm basically teaching myself qiime and R. So I've finally made it through qiime and have uploaded two files into R. My OTU table us…
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Would be nice for people to be able to do their taxonomy assignment within R, and since its not available perhaps it should be added to the dada2 package.
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Hello,
I'm wondering if there is a recommended approach for dereplication and clustering of reads in pooled specimens. Here's the specific problem I'm trying to address (forgive me for the extremely …
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I ran SURPI to test it on a small sample comprising 200k already prefiltered Illumina reads (93MB fastq). I know there are viruses in there... Kraken spots perfect 31mer matches for a handful of RefSe…
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I first would like to thank you for the effort to provide the community a reliable composition based metagenomic taxonomic classifier. On samples with many unknown organisms the similarity based appr…
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For low numbers of denoising-results, the following might work (against NR database)
``` S
annotate::blastSequences
```
For larger datasets with hundreds or thousands of species-genotypes, this mig…
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What if anything is permitted or prohibited by the current DwC about the DwC class hierarchy ?
On 8 Aug 2015, Bob Morris raised this on the TDWG-tag mailing list and this issue represents an attempt …