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I would refer to simple principles rather than too specific terms when it's not necessary, especially if the target audience includes non specialists. For example, contrasting closed vs open reference…
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Hi there,
I noticed that we are having an issue where barcode 1 seems to be missing for alot of the reads, which reduces the cell counts and reads per cell. I'm wondering if this may be due to us u…
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In a small set of 12 samples and a small single-gene amplicon panel I see 7+ bins in my reference with zero log2 and zero spread. How is that possible? Coverage is >400x. All samples have good coverag…
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I am using a conda installed cnvkit 0.9.8 environment (and this has also been tested on a conda installed cnvkit 0.9.7 environment, with the same result).
_cnvkit.pl batch_ runs successfully, produ…
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0 paired-reads (in 0 unique pairings) successfully merged out of 541 (in 3 pairings) input.
0 paired-reads (in 0 unique pairings) successfully merged out of 100 (in 1 pairings) input.
0 paired-reads…
Tr1sh updated
4 years ago
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Dear Kristoffer,
I'm very impressed with isONclust's performance - thank you for designing this much-needed tool!
Out of curiosity, I'm evaluating its utility for clustering reads from ONT genom…
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My apologies for this likely being a naive question. I have performed a series of amplicon sequencing of virally expressed genes with sequence-specific barcodes. I have used Dada2 to perform filtering…
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Dear all,
I'm working on differential abundance with my 16S amplicon sequencing data and to do that I followed the example documentation on this topic ([http://bioconductor.org/packages/release/bio…
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Hi,
I have installed Nanoplot on our cluster running linux inside a clean conda environment called nanoplot:
```
conda install -c bioconda nanoplot=1.32.1
````
I then tried to test it using a …
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Thanks for your help. This issue could be cancelled if it's fine with you.