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[Suggestion to add amplicon resequencing data-processing functionality, brief description of new scripts to aid in this, and request for guidance on unit testing or code review]
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## Justification …
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I saw there was a placeholder file for Gateway cloning and thought to give it a go. Did you have any idea on how to do it @BjornFJohansson?
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I'm using geminimulti and pisces on some targeted amplicon sequencing, but each run might contain a different mix of amplicons located around the genome so I can't easily use the chromosomes option to…
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Hi, I'm running DeviCNV with BAM files from 60 samples, and the very first step "STEP1_calculateReadsDepthOfAmp" is taking ~3 days per sample. Each BAM file is around 2 GB. Is this runtime to be exp…
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### Ask away!
Hello,
I'm trying to run some older data through the wf-artic pipeline. I used Guppy to basecall this data with model
dna_r9.4.1_450bps_sup.cfg.
![image](https://github.com/u…
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I tried haplochecker with my own alignments (long PacBio amplicons) and got the following error. To test whether it due to our data, I downloaded the [HG96 test data](https://github.com/seppinho/mtdn…
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Hi,
Thanks for creating and maintaining this amazing fieldsbioinformatics.
I stumbled across this silent bug when running a new primer scheme for MPXV (spiked in yale scheme). The `primer.bed` f…
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I am trying to understand how to adjust -k since it seems to impact the results dramatically.
For example with primers of length 25 and a -k of 15 I get no amplicons.
I think this is the same as cha…
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Hi.
I have applied the program in WGS data of several cell lines. I started from .fastq files and used PrepareAA.py to generate CNV calls. However, all of these runs generated empty AA_CNV_SEEDS.bed…