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Hi Tao,
We have a submission for Will Greenleaf’s lab that I worked on for a while now. It’s a bit more complicated and I was wondering if we could have a call this week and we go through the sprea…
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Hi,
Congrats on the release of this package. It seems that this tool will be quite useful for the CRISPR community. I especially like the functionality for accounting for heterogeneity in editing e…
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Specifically, why doesn't the following line equate to TRUE for all indices in `sceptre_object@grna_assignments`:
`sceptre_object@grna_assignments$grna_group_idxs[[pert]] %in% sceptre_object@grna_ass…
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When I try to create a sceptre object with the example code in "The Whole Game"
```
# load the data, creating a sceptre_object
directories
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I am dealing with high-MOI data, and the number of non-targeting cells is very small. I am wondering if sceptre can do DEG analysis between cells containing a given gRNA and all other gRNA negative ce…
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Hi,
I am currently using sceptre version 9.1 and while running the calibration I encountered an error (see below) and the calibration is not performed.
Running calibration_check in parallel. Chan…
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Hello,
When I try to run `construct_cis_pairs()`, I get this error message:
```
Error in `dplyr::select()`:
! Can't subset columns that don't exist.
✖ Column `grna_target` doesn't exist.
```
…
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Hi JP and Team, really enjoying this set of tools.
I've run into an error from the `addEditingWeights()` function that occurs when the targeted sequence doesn't have any nucleotides with values gr…
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Hi,
I am suddenly getting this error when I try to run `set_analysis_parameters()`
```
Error in convert_covariate_df_to_design_matrix(covariate_data_frame = sceptre_object@covariate_data_frame,…
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Hi!
As far as I understand, in QC step, for a given gene-gRNA pair, sceptre removes cells (both ctrl & perturbed) where the target gene count is 0.
Our team found that for our dataset, there is a …