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I'm bulk RNA-seq data and sc-RNA data with Ensembl Ids. And using cell type Fibroblasts.
```
> dim(bk.dat)
[1] 546 19988
> dim(sc.dat)
[1] 1159 19828
> sort(table(cell.type.labels))
…
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Hello.
I tried to reproduce the [MAESTER publication](https://github.com/petervangalen/MAESTER-2021) but I hit some issues on having too many open file limit on [a scRNA-seq dataset](https://trac…
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Thanks a lot.
I am learning seurat v5.
```
rm(list = ls())
library(Seurat)
library(SeuratData)
library(SeuratWrappers)
library(Azimuth)
library(ggplot2)
library(patchwork)
options(future.g…
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I have curated multi-gene displays for all datasets in the following profiles (very few have issues and I am keeping track of them and will create tickets later):
- Adult
- Aging
- Central auditory…
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@sqjin
I am working on CellChat-vignette in your tutorial.
when I run `cellchat
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Hello,
I am trying to map a list of fastq files to reference mus musculus for my research. My system has 32GB ram and i7 processor. But when i start running i get the following error. I don't have an…
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Hi, I have been using kb-python to analyze scRNA-seq. first, thanks for creating such a user-friendly and useful tool. I appreciate it! I just had a technical issue when reanalyzing scRNA-seq in [this…
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Hi, all
When I use monocle3 default parameters to `plot_cell_trajectory` , I got a not fully connected graph, as below:
![image](https://user-images.githubusercontent.com/32219336/53737415-70006980…
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https://mp.weixin.qq.com/s/GRhEn7TumxjOoQbD_ixsdw
ixxmu updated
4 weeks ago
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Hi,
first of all, thank you for this tool. It has really saved our ass in a different experiment where HTO-based demultiplexing failed. Thank you a lot.
I am running now vireo on two 10x sequenc…