-
Hello,
I am using Canu for the first time and trying with the quickstart data. I have followed the commands are described in readthedocs but the following is happening:
```
hjb60@hjb60-Virtual-Bo…
hjb60 updated
6 years ago
-
I’m trying to use vg for metagenome analysis. As many assembled fragments have attached to the reference sequence with only in one end (and other terminal of the sequence has not determined), so I cre…
-
I guess the input to the script would be:
1. the list of node IDs, created by the viewer application
2. some source of DNA for nodes in the assembly graph in question. for GFA/LastGraph files I gues…
-
I am using canu 1.7 for assembling a genome of ~1.4gb size with ~70X pacbio data.
canu -p canu -d canu-pacbio useGrid=false maxThreads=60 maxMemory=500 genomeSize=1.4g -pacbio-raw file1 ....
I hav…
-
Hi, I am assembling mouse genomes with PacBio Sequel data.
I am running in a similar issue like #417, however, removing the correction/1-overlapper/results/ and re-run canu did not solve my problem…
-
Hi,
I'm having a similar issue like #546: https://github.com/marbl/canu/issues/546
The assembly step stops with the following error:
```-- Canu 1.6
--
-- CITATIONS
--
-- Koren S, Walenz BP, Be…
-
Hello Canu team,
I'm trying to run canu to assemble a 14 MB genome with MinIon reads.
It works find for a couple of samples, but in two of them I get this:
```
-- Canu release v1.5
-- Detect…
-
I generated a *.gfa file from an alignment in vg. When I run:
reveal bubbles -r ref test.hal2vg.gfa >test.hal2vg.bubbles
Traceback (most recent call last):
File "/usr/local/bin/reveal", line 1…
-
According to gfa-spec, L-line in GFA format looks like:
`L `
In CANU(v1.5), the CIGAR string takes `` as query, and `` as reference.
Would you mind considering swaping them as https://github.…
XLuyu updated
7 years ago
-
Hi again,
I am working on assembling a 3.3 Gb invertebrate genome using about 50X PacBio coverage (160 Gb reads). With your suggestions for specifying grid options (Issue #886) I was able to get th…