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**Copy and paste the exact command you tried to run**
``` bash
apptainer exec --bind /gpfs/shared_data/references/Homo_sapiens/NCBI/hg38:/mnt \
/gpfs/shared_data/singularity/flair/flair_2.0.0.sif…
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### Ask away!
I am using wf-transcriptomes workflow for analysis my ONT pilote transcriptome data which is from only 1cDNA library. After I run the following command, the result I got are the html re…
wuy24 updated
11 months ago
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Hello,
I'm having my very first experiment with flair for my ONT data. But while running the collapse module, I'm facing the issue when the *.isoform.gtf somehow keeps returning isoforms the same as …
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Dear @vdemichev,
First of all, thank you very much for the great software! I have used DiaNN before with its GUI on a Windows machine. However, I need to set up a linux based pipeline now and am ru…
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**I have read the paper (https://doi.org/10.1038/s41467-020-15171-6)
and the manual (https://flair.readthedocs.io/en/latest/) and I still have a question about**
- collapse
I am using flair t…
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Hello, I'm running Sqanti3 with the following code:
![image](https://user-images.githubusercontent.com/70981989/165827091-b3de4ac3-13d4-4c84-8dd0-277274ccdf74.png)
but for some reason the progr…
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Hello, FLAMES aligns my reads to the reference genome but during realignment I get this error:
```
### skip aligning reads to genome 2023-12-07 15:57:35
### read gene annotation 2023-12-07 15:57:…
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Hello, Dr. I have another question. This is my code. I can make sure there is multiple isoforms. However, it does not display. Could you help me? Thank you!
```
bigWigs
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I am working on a project that utilizes BRAKER to annotate recently assembled genomes for wild plant species. During the course of the process, we became concerned that stringent RepeatMasking may res…
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Hi, Aaron
Your project has greatly addressed my issues, and I sincerely appreciate it.
However, can your software run in multithreaded mode to improve its operational efficiency? Because my Fas…