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Hello
I would like to ask if BLAZE can work with other type of single cell data such as split-seq technology.
These barcodes are a bit different than the 10X ones, since this technology uses 3 diff…
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Hi,
Will the current version of dorado take barcodes into account when identifying duplex pairs, or does this need to be done separately and provided with the `--pairs` option to `dorado duplex`?
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Hello,
I found an issue where the barcoding_summary.txt generated by `dorado demux` is not working properly while using the --no-classify. I'm running the barcoding from the `dorado basecaller` ste…
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### Ask away!
The PCR-cDNA barcoding kit, incorporates UMIs with the strand switching primer into the strands which are then sequenced. Is there a point in this pipeline that takes these UMIs into ac…
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I am super keen to try gibpss! Any possibility that you might add support for bar codes on the 1st and 2nd reads that in combination are the unique identifier?
--Rob
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I would like to build some custom models using bonito. As input, I would like to use reads from samples that were barcoded. Is there a standard way of doing this?
Based on information that I could …
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- Link to preprint
- Calculator for barcoding complexity
- Cost / reagent usage spreadsheet
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# Background
SBO is made up of 9 different work groups spread across 4 separate locations.
- Greg G. has worked on a timeclock prototype platform
- He has thought that minimum 9 hardware devices …
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I have version 0.5 of poreplex installed both on a work server (linux) and on my own Mac. I am unable to get either of them working.
If I run poreplex on the sever (python 3.7.2), when I try to use…