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Hi ShouWen,
I'm wondering whether there is a simple way to add max_cells or max_molecules to the config files used for the MATLAB or python-based DARLIN pipelines. Tuning the read thresholds is usefu…
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Hi,
Is there a way of matching a detected virus with the barcode of the cell from which it originated?
Thanks and good day.
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Hello Dr. Li,
I have been processing TCR data in single cells recently. I observed from the TRUST4 output file that the numbers after the barcode are not as ordered as 0123.
CATCGAAGTTAGGGTG_0 0 TR…
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Hi all,
I am working on an (nf-core) pipeline (either a separate pipeline for single-cell long-read DNA barcoding. Or to include it in the current https://nf-co.re/scnanoseq/1.0.0/ to support diffe…
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## Problem Summary
Several values in HuBMAP metadata require consolidation or mapping updates to align with the current standards. The issue affects two categories:
- **Granularity Mismatch Mapp…
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I'm really impressed with the speed and usability of NEBULA, great work! My data set has barcoded cells that are unique to each classified group, as such, I'd like to be able to incorporate clonal bar…
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Hello,
I have a combined seurat object (called "seuratobject2") consisting of 20 different patient samples whose barcodes (orig_barcode column) are like below:
`head(seuratobject2@meta.data$or…
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Hello
I would like to ask if BLAZE can work with other type of single cell data such as split-seq technology.
These barcodes are a bit different than the 10X ones, since this technology uses 3 diff…
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Hi! Excited to try out this tool.
I currently have cell barcodes with corresponding cell-type annotations but the cells/barcodes that were filtered out when I processed my data in Seurat are still …
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Hi, I’m just starting out in this field.
We have an scRNA-seq dataset that includes 438 million paired-end reads (2x150bp) from approximately 8000 cells sourced from tissue samples. We processed the …