-
Some questions
* Will FACS sorting disturb RNA?
* How should we preserve the cells post-sorting and pre-RNA isolation?
* How should we isolate RNA?
* What markers should we sort on?
-
**Project UUID:** ae71be1d-ddd8-4feb-9bed-24c3ddb6e1ad
**Project Title:** Single-cell RNA-seq analysis of human pancreas from healthy individuals and type 2 diabetes patients
**Project Short Name:**…
-
Project UUID: 091cf39b-01bc-42e5-9437-f419a66c8a45
Project Title: Profiling of CD34+ cells from human bone marrow to understand hematopoiesis
Project Short Name: Human Hematopoietic Profiling
Submi…
-
Hi,
Has anyone tried Demuxlet on single nuclei using 10x? Does it work?
Also, what are people using for genotyping of human samples?
-
**For which schema is a change/update being suggested?**
enrichment_protocol.json
**What should the change/update be?**
New FACS module that could be included in enrichment protocols. This mo…
-
```
I think we all agree that having a means to record markers used in FACS sorting
of cells is an important and useful. But there is an ongoing issue in CL with
species specificity and these axiom…
-
One point of uncertainty: whether they actually used the two different epitopes (KK10 and KK10-L6M)
for the tetramer (pentamer) sorting, or just the KK10. I cannot make it out from the methods or the…
-
### Project short name:
LethalCovidLungAtlas
### Primary Wrangler:
Wei Kheng
### Secondary Wrangler:
### Associated files
* Google Drive:
https://drive.google.com/drive/folders/1PVVRZ3OiNyBI79lF…
-
This is a great tool! Thank you for development. Can you please generate/provide similar T subset resources for mouse datasets? It will be greatly useful as many labs including ours generate CITE-seq …
nroak updated
2 months ago
-
### Project short name:
SARS-CoV2ControlHostGenes
### Primary Wrangler:
Ida
### Secondary Wrangler:
### Associated files
* Google Drive: [folder](https://drive.google.com/drive/folders/1vcfeLlvtjD…