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Dear Brian
I have a problem about mapping and aligning transcripts to the genome using PASA. Can the transcript here be a non-coding transcript? My purpose is to verify the GT-AG rule for non-coding …
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Dear
I am trying to do allelic analysis Hic data getting same error G2 is mapping reads are zero and iced normalisation step getting error. I used bellow command to run analysis. non allelic analysi…
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Hello, I like very well AutoHiC with 3d-dna, but I want to improve your assembly pipeline using YAHS but I have some problems.
I already try to run onehic with peanut example to test the pipeline …
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Hi, Felix.
I'm so sorry to bother you again. I have WGBS data from the same batch for two species (Accel Swift data) and have already trimmed it using the method you suggested, with the following c…
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@jeizenga
hello, I read about issues with mapping long reads for pan-genomes, so I was wondering if the new version of vg giraffe supports mapping long reads.
[https://github.com/vgteam/vg/…
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I used below command to map one wheat genome against reference in a 32 cpu and 1TByte machine.
minimap2 -x asm5 -f100 -K4g --cs=long -t 32 --secondary=no iwgsc_refseqv2.1_assembly.fa arinalr_sm.fa…
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Hello, When I used the GCI software to assemble my T2T genome, the following error occurred. What happened?
Used arguments:{'reference': '/export/home/zzl/YQ/Genome/Mel.genome.fa', 'hifi': ['/exp…
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Search for a GO term in the data portal should use a mapping file to check mapping files since the GO terms aren't stored directly in the data files.
The KEGG -> GO mapping file is https://www.geno…
aclum updated
12 hours ago
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Hi,
Thank you for developing this tool for evaluating L1 expression. I'm encountering an issue while running L1EM from a conda environment. Here is my setup and the steps I followed:
**Environme…
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3 genomes are offered for each species. This is the result of soft, hard and not masked genome versions. Not masked and soft-masked genomes are identical for purposes of transcript mapping (repetitive…