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The current delta-cq vignette shows how different forms of qPCR data can be incorporated and analysed by tidyqpcr but is not a good example of plate design according to the tidyqpcr ethos. A new vigne…
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Check the MIQE-recommended GeNorm method, and compare in detail to tidyqpcr's `normalizeqPCR` function, which is geometric (Ct/log-scale) and uses median.
Method is described in [Vandesompele et al…
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```
What version of Prat are you using?27651
Do you use embedded libraries or standalones?Embedded
What language/realm type?enUS - RP
What steps will reproduce the problem?
1. Unsure - I have it, S…
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Add capability to import [RDML (Real-time PCR Data Markup Language) format](https://academic.oup.com/nar/article/37/7/2065/1017981) files.
Try using the [RDML package](https://academic.oup.com/bioi…
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https://miqingwang.github.io/2021/01/11/%E3%80%90Android-%E5%BC%80%E5%8F%91%E6%A1%86%E6%9E%B6%E6%90%AD%E5%BB%BA%E3%80%91-%E5%AD%A6%E4%BC%9A%E4%BD%BF%E7%94%A8MVVM%E6%9E%B6%E6%9E%84%E7%9A%84%E6%90%AD%E5…
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Currently, the conversation algorithm seems to lack a research(mathematical) background. It just follows some kind of pattern.
I think the conversation algorithm need to be based on some decent res…
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https://miqingwang.github.io/2020/12/15/%E3%80%90Android-%E5%BC%80%E5%8F%91%E6%A1%86%E6%9E%B6%E6%90%AD%E5%BB%BA%E3%80%91-%E5%AD%A6%E4%BC%9A%E4%BD%BF%E7%94%A8MVP%E6%9E%B6%E6%9E%84%E7%9A%84%E6%90%AD%E5%…
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Write a short email that can be forwarded by eLife open innovation colleagues and others, inviting people to use/test/contribute to tidyqpcr.
Intro needs to be 3 sentences.
Perhaps we can include …
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I would like to request a galaxy tool based on either the geNorm or NormFinder (preferably geNorm) algorithms which serve to determine the most stable housekeeping genes for qPCR analysis.
Both are p…
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tidyqpcr is intended to be
a) a user friendly and flexible tool for analysing qPCR data and
b) an educational tool for users to learn how to conduct MIQE-compliant qPCR experiments and also
c) a …