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Hello! So I’m trying to use ShortStack for miRNA identification from sorghum roots samples. In order to test the efficiency of our small RNA library protocol we initially sequenced a small pool of 20 …
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The Biological Roles of microRNAs in Drosophila Development
PMID: 39057224
Check annotation of miRNAs mentioned have been curated and potential template for GO-CAMs for general processing
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I've tried to this program but somehow didn't get any results, to troubleshoot, I've used miRNAs from literature that should have worked (i.e. miR173) for Arabidopsis. Note that I downloaded the trans…
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First of all, great program! Very easy to use and flexible.
I've been running the pipeline on a brown algae and I get 2037 predicted miRNAs! But most of them have a lot of reads mapping to the loop…
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Hi, I found merged miRNA in the annotation file, but they are in different locations of the genome, according to what criteria is merge done?If I want to count separately, can I assign expression valu…
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I am trying to identify miRNA from my data. In my data, I knew apriorly that sample contains novel miRNAs and I am also getting some of them when using mirdeep2/mirdeep* sequence aligner. But I am not…
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Hello!
I have been using TOGA lately for transferring hg38 gene annotations to a newly constructed genome and it looks good so far! However, I do notice that annotations for non-coding RNAs such as…
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We need to include HGNC Ids in the GPI file alongside the mature miRNAs that derive from the precursor miRNAs that those HGNC Ids map to. This will help the AmiGO users to search for miRNAs.
Need t…
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Dear author,
It's fancinating to integrated all kinds of miRNAs' resources.
The internet based will highlight the query speed, especially for researchers in China.
So, are there local databas…
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### Description of feature
As discussed in slack with @sdjebali and @lpantano it will be nice to add this feature to the pipeline. I copy the discussion here:
@sdjebali:
> I want to produce a singl…