-
Hello @kimrutherford and @ValWood, first congrats on managing to pass the submission pipeline!
I noticed that the new submission no longer includes the mitochondrial chromosome sequence. I know tha…
-
This Ensembl pipeline is probably one of the largest sources of incorrect IEA annotation.
One major issue that could be fixed is that the relationships appear to be ignored.
So in this case…
-
New GO arrangement
iron-sulfur cluster assembly complex (GO:1990229)
|_cytosolic [4Fe-4S] assembly scaffold complex (GO:1904564)
|_cytosolic [4Fe-4S] assembly targeting complex (GO:0097361)
…
-
Hi,
we recently sequenced a batch of 19 fungal isolates. I tried to assemble the genomes a few different ways (simplex + duplex or simplex corrected only) in order to figure out if there is still a…
-
One thing thet really called my attention was the lack or chloroplast/mitochondrial assembly tools in Galaxy. Recently, Novoplasty was included, but I feel others could be added.
I would recommend:…
-
Hi,
I am trying to assemble the genome of a worm, and I was hoping that minipolish would detect the mitochondrial genome and output it in the gfa file.
I have sequencing one of my worm species a l…
-
Hi,
Now I want to incorporate RNA-seq via PASA when conducting the genomic gene annotation. When I used the FastQC for assessing my Illumina RNA-seq data, I found there were many overrepresented seq…
-
Hi, I am trying your method to construct the mt genome, it is very fast which surprised me, My fastq data quality is not very good, I found the final result has multiple scaffolds and many same genes …
-
Hi,
I am trying to correct a mitochondrial genome assembly, I thought it would be simple and straightforward, and I got an error:
I created a new conda environment, no issue during installation, b…
-
Hello,
I would like to assemble the plant mitochondrial genomes using Novoplasty. I read your recently published article, mentioned that RuBP used as a seed sequence for cp genome. But I would like t…