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One thing thet really called my attention was the lack or chloroplast/mitochondrial assembly tools in Galaxy. Recently, Novoplasty was included, but I feel others could be added.
I would recommend:…
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Hello,
I am trying to use NOVOPlasty to assemble an hexapod mitogenome from 150 bp paired reads. Contig is fine until a point where a 79bp sequence is indefinitely repeated at the end of the contig…
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hello,I am trying to use this software to assemble the chloroplast genome, and the result shows INVALID SEED, PLEASE TRY AGAIN WITH A NEW ONE. I want to know where mistake happened, DoIyou have any s…
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Hello!
I am assembling a beetle mitochondrial genome, using this [mt genome](https://www.ncbi.nlm.nih.gov/nuccore/NC_038115.1) from the same beetle family as the seed. It appears mostly assembled, …
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Hello,
I used Novoplasty on metagenomic data. I used this file as a configuration file:
============
Project:
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Project name = MS_assembly_chloroplast
Type …
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Hi,
I am trying to assemble three plant mitochondrial genome sequences using Novoplasty. I successfully assembled two mitochondrial genomes. But When I try to assemble the third genome sequence usi…
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**Plan for your class project:**
We recently generated Illumina shotgun sequence data for the non-model plant organism (my study system), Geranium maculatum. I plan to evaluate genome structure with …
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I am assembling turtles mitogenomes and in some cases the output I obtain is a "Circularized genome", actually made up of 2 non overlapping contigs: in all these cases, the interruption is in correspo…
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I am new to perl. Using Strawberry perl on Windows, I have been trying to assemble the mitochondrial assembly test dataset you have provided, but keep getting this error message: "Can't open file Homo…
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I have two runs of the same Illumina library and merged them using 'cat' prior to NOVOplasty. The first run's set of reads did not assemble a mitogenome, so we sequenced a second run of same library w…