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Hi,
I have Pacbio FLNC reads in fastq format. What options should be specified while running the tool. I was thinking
`--data_type pacbio --fl_data`. Is this correct?
Thanks
Abhijit
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We're just getting in our first standard 16S data from the PacBio Revio which is using the [Kinnex kit](https://www.pacb.com/technology/kinnex/), and will soon be getting 16S-ITS-23S data. I know the…
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Hello,
I am currently trying to run finder on three whole genome samples:
1. Sequenced with Illumina HiSeq x ten
2. Sequenced with Illumina Novaseq 6000
3. Sequenced with PacBio SMRT
Samples…
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My problem is that I am having difficulty obtaining a list of variants using bcftools mpileup and bcftools call with PacBio data. I do not have this problem with data of Illumina paired-end sequencing…
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Hi,
I have been trying to analyze my sequencing data that come from an AVITI platform instead of Illumina. The FASTQ quality score are higher than 41, which results in an error when I run the itsxp…
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Hi!
Is there a chance it will work with long reads like Nanopore or Pacbio?
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Hello @giovanniquinones,
I have been trying isoLASER out but I'm failing to generate the output pickle files from:
`isolaser_extract_exonic_parts -g {annot.gtf} -o {transcript.db}`
For the ann…
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Hi all,
I am new to the field and I'd like to verify my variant calling pipeline using latest CHM13 as reference. I have the HG002v1.1.fasta here and I got the truth set v1.1.vcf from NIST.
> (ht…
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Hi,
I am trying to use genomescope to estimate heterozygosity and genome size of a fairly large plant genome, ~ 3.65Gbp. I am using the histogram of canu-corrected reads. Here is what I am getting:…
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Hi
I am trying to use the metamos software to analyse some pacbio data. When I try to run the pipeline I get this message:
Starting Task = preprocess.PREPROCESS
**\* metAMOS: Error: cannot correc…