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Using various strategies we can determine the expected size of an analytical PCR amplicon based on the parts list. Options for this:
1. Crude, but low user demand: assume mID-derived part lengths a…
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I get the following issue whenever I try to run pcr.seqs with a custom region from the UNITE database:
[ERROR]: seqs are not aligned. When using start and end your sequences must be aligned.
I c…
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Currently, Analytical PCR is a Dashboard task but is handled differently than other tasks; it doesn't have a Status "ready to queue analytical PCR" message. Golden Gate g and Minipreps m both currentl…
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Hi folks,
I just opened https://github.com/nokia/TPMCourse/issues/22, because I think the example they include of sealing data in nvram with a PCR policy is misleading. I'm opening a related issue…
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Analytical PCR tab currently has "recommended oligo" code (for 2 primer pairs) that works ok for our specific Golden Gate construct set but doesn't scale to other GG sets or handle GGs that don't have…
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The "Sealing" section of nvram.md demonstrates the following command sequence:
```bash
$ tpm2_pcrread -o srtm.pcrvalues sha256:0,1,2,3
$ tpm2_createpolicy --policy-pcr -l sha256:0,1,2,3 -f srtm.p…
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## Feature Request
Seal the LUKS encryption keys for the `EPHEMERAL` partition using a TPM register that depends on confidential information from the `STATE` partition.
### Description
Consider a…
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Hi all,
I'm using the barcoding kit SQK-NBD114.24 for sequencing on MinIon Flow cell. Before the library preparation and sequencing, I'm doing on each samples a pcr with barcoded primers:
![image]…
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We should add observability for the replication lag in PCR (current time - replicated time, where the src and dst cluster are exactly the same). Since replication lag is essentially the RPO for custom…
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Is pychopper compatible with with the new SQK-PCB114.24 kit. Will the '-k PCS111' work ?