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In the Chronos 1 paper, non-expressed genes are used to define the negative control sgRNAs for each line. Is there a way to do the same in Chronos 2? As best as I can tell, that can only be done on a …
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Hi all, thanks for developing this very useful tool.
I have a question on how the clinvar variant significance annotation table is matched to the generated guides. I've attached a subset of my out…
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Hi, I encounter an error at the very last step during the design of sgRNA for CRISPR activation.
Everything is going fine until I try to add the CRISPRai scores.
After typing the command line in R…
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Hello
I am using GECKO library and I was wondering how I can get a sgRNA library file for the input.
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Hi,
I am trying to design sgRNA for campylobacter, but realised the campylobacter genome sequence is not available in the shiny app. Is there a way to upload my own genome sequence into the app? …
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When I tried to run the tutorial script available from CRAN, the function run_sgrna_count() could not be found. Could you try and fix this issue? It'd be great to try your package on pooled CRISPR scr…
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1) Search for "EGFR"
2) Clicking on sgRNA / Gene Expression doesnt return results
![image](https://user-images.githubusercontent.com/19891619/70996244-5d46d200-20a0-11ea-93d7-bfddd950a05e.png)
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Hello! I'm trying to use crispy to make a CNV correction of my CRISPR/Cas9 KO screen data, but first I wanted to run the example script, mostly to get a feel for the package and check that everything …
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**Analysis of tested guide RNA’s used for gene editing via CRISPR to predict optimal future guide sequences**
Despite the apparent simplicity of this sequence matching strategy to target the CRISPR…
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For some ENST numbers and HGNC we run into the error `AttributeError: 'str' object has no attribute 'iteritems'` (whole error at the bottom). This happens because sometimes the `edit_map` becomes an e…