Often, it is not even clear whether a given cluster is uniform in unsupervised scRNA-seq data analyses. Here, we proposed the concept of cluster purity and introduced a conceptually novel statistic, named ROGUE, to examine whether a given cluster is a pure cell population.
Installing dependency package
Before installing ROGUE, the “tidyverse” package should be installed first:
install.packages("tidyverse")Installing ROGUE
To install ROGUE, run:
if (!requireNamespace("devtools", quietly = TRUE)) install.packages("devtools")
devtools::install_github("PaulingLiu/ROGUE")For more details and basic usage see following tutorials:
The scripts for producing all the quantitative results in our manuscript can be found in scripts.
If you use ROGUE in your research, please considering citing:
Please contact us:
Baolin Liu: pauling.liu@pku.edu.cn
Zemin Zhang: zemin@pku.edu.cn
©2019 Baolin Liu, Chenwei Li. Zhang Lab. All rights reserved.