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primer3
Primer3 is a command line tool to select primers for polymerase chain reaction (PCR).
GNU General Public License v2.0
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SEQUENCE_PRIMER_PAIR_OK_REGION_LIST doesn't honor specified values
#81
kubu4
opened
1 day ago
0
Update usage info to match ntthal defaults
#80
emmcauley
opened
1 month ago
0
ntthal set defaults are different than ntthal usage information defaults
#79
emmcauley
opened
1 month ago
0
Major Prformance Improvements for thal
#78
DougTownsend
opened
3 months ago
0
ntthal negative Tm calculations
#77
cesimsek
opened
4 months ago
1
Initialize thermodynamic parameter tables to default values
#76
DougTownsend
opened
5 months ago
0
Issue when make test
#75
nanu4github
opened
6 months ago
0
Question: How PRIMER_MISPRIMING_LIBRARY works?
#74
harazono
opened
1 year ago
0
`max_loop` not working when calculating hairpin structure?
#73
dodoflyy
closed
1 year ago
2
A question about picking sequencing primers
#72
yangjw1996
closed
1 year ago
2
Abnormal primer positions
#71
shiyi-pan
opened
1 year ago
2
Understanding the dpal logic
#70
karen-nativ
opened
1 year ago
0
Add CI workflow for Ubuntu ARM64
#69
martin-g
opened
1 year ago
7
Question: Is ARM64 architecture supported ?
#68
julien-faye
closed
2 years ago
2
What PRIMER_PRODUCT_OPT_SIZE is needed for?
#67
DmitriiSukhomlinov
closed
2 years ago
2
Fix unhandled errors of malloc
#66
Thomas1664
opened
2 years ago
0
Signal 11 segmentation fault in thal.c
#65
InvisOn
closed
2 years ago
2
A question about ntthal to cacualte "ANY" bounds between two oligos
#64
zenghp88
opened
2 years ago
0
Penalty for template pair mispriming is perhaps calculated twice
#63
Tarasovk49
opened
2 years ago
0
Final record not terminated by '='
#62
Miduoduoduoduomi
closed
2 years ago
4
unable to open file /opt/primer3_config/stack.ds
#61
Hytham79
closed
2 years ago
7
Segmentation fault
#60
Princysaini
closed
2 years ago
1
Scoring pre-selected primers?
#59
joshuak94
closed
2 years ago
3
critical memory error
#58
jocelyne8
closed
2 years ago
4
critical memory error with humrep_and_simple.txt as mispriming library
#57
jocelyne8
closed
2 years ago
3
Could primer3 design primers based on alignment
#56
starsareintherose
closed
2 years ago
3
thal.c caused crash of build on ubuntu, "/0" needs to be just zero
#55
sebogawa
closed
2 years ago
1
thal.c caused crash of build on ubuntu, "/0
#54
sebogawa
closed
2 years ago
2
how to determine the integer input for SEQUENCE_OVERLAP_JUNCTION_LIST
#53
juechenyang
closed
2 years ago
3
Possible BUG: No triloop and tetraloop bonuses for entropy?
#52
Oleg-Mozhey
closed
2 years ago
1
Primer3 issue with the Sequence_ID
#51
Kisekya
closed
2 years ago
3
What is the difference between primer3web and primer3Plus ?
#50
ptranvan
closed
2 years ago
1
Fix out of bounce read in oligotm
#49
Thomas1664
closed
2 years ago
2
When is the next release of Primer3
#48
nh13
closed
2 years ago
3
How to set the SEQUENCE_TARGET or SEQUENCE_EXCLUDED_REGION parameter?
#47
sen1019san
closed
2 years ago
1
Stray text NOWWW in manual
#46
peterjc
closed
2 years ago
1
Multiplexing assays takes a lot of time
#45
haseebz
closed
2 years ago
2
Nested structure for primer results
#44
peterjc
closed
3 years ago
1
Question re TEMPLATE_MISPRIMING parameters
#43
genya
closed
3 years ago
1
Added SEQUENCE_OVERHANG_LEFT and _RIGHT
#42
wholtz
closed
2 years ago
1
number of 'ok' primers is sometimes negative
#41
wholtz
closed
2 years ago
1
Fix MAX_POLY_X calculation
#40
wholtz
closed
2 years ago
1
poly_x calculation failures
#39
wholtz
closed
2 years ago
0
Question on calcHairpin dg discrepancy
#38
sooheelee
closed
2 years ago
3
Primer3 not finding microsatellite primers from reference genome
#37
ctdarwell
closed
2 years ago
1
The salt ion correction formula in file thal.c is inconsistent with that in file oligotm.c
#36
Wy2160640
closed
2 years ago
1
Shouldn't stackmm value for TA_AA be 4700 instead of inf?
#35
Curley-Joe
opened
4 years ago
2
Add batch mode option to ntthal
#34
Frederooni
closed
4 years ago
0
Crash in check_primers if primers are perfect match
#33
Curley-Joe
closed
2 years ago
3
Return all primers for pick_sequencing_primers task
#32
shunhuahan
closed
2 years ago
1
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