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Hey there
does this tool support Direct RNa seq data? i ran Direct RNA sequencing using SQK-RNA004 library kit.
thank you
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Hello,
this repository belongs to the publication "Deciphering transcript architectural complexity in bacteria and archaea" (https://doi.org/10.1128/mbio.02359-24) . You mention in the publication'…
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Hi,
I haven't found a similar issue, so apologies if this has already been addressed.
The output of f5c tsv resquiggle appears to add an additional tab at the end of the line in cases where ther…
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Hi, I'm trying to perform a m6a (on RNA) detection using tombo (and some other softwares).
I'm having trouble performing the resquiggle, I've read many of the other opened issues and I can't find a …
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So, I performed direct RNA sequencing on nanopore and got fast5 files for them. When I do tombo resquiggle, should me reference fasta file by DNA or RNA sequence in fasta?
For instance, if I sequen…
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Hi,
Does the capTEs suitable for nanopore direct rna sequencing data?
Thanks,
Bai
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Hello,
I am interested in direct-RNAseq data analysis. In the introduction, you have specified the used sequencing kit as "SQK-LSK108", which I believe is a cDNA or gDNA kit and not a dRNA kit. Cou…
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It is clear that eventalign-dependent modules does not support R10.4, but it looks like polya uses non-eventaligned durations. Does this mean that polya should be fine to run on R10.4 data, or should …
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I really like your preprint and I would love to use it for direct RNA sequencing.
Do you happen to work on adjusting the UNCALLED for direct RNA sequencing? Given that an accurate RNA k-mer model is …
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Hi,
I am trying to run eventalign on reads from RNA004 and I get the following error:
[meth_main::13.631*0.41] 0 Entries (0.0M bases) loaded
[pthread_processor::13.631*0.41] 0 Entries (0.0M bases…