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Hi,
When working with metabarcoding data where you're not interested in any specific taxonomic group but want to quantify general diversity, you almost always have a mix of different genetic codes …
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Hi @hoelzer,
First, thank you for designing this nice tool.
I want to use poseidon in a set of mitochondrial genes, but, apparently, poseidon only uses the Standard Code.
Is there an option to de…
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Hi,
I am trying to assemble the genome of a worm, and I was hoping that minipolish would detect the mitochondrial genome and output it in the gfa file.
I have sequencing one of my worm species a l…
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The GRCh38, T2T-CHM13, chimp alignments fail to align more than half of chrMT despite having identical sequence to
GRCh38. This is the t2tChm13_20200727 assembly
[chrMT.pdf](https://github.com/…
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Hi! I am having some trouble filtering my RNASeq reads. I have a lot of mitochondrial rRNA that I want to get rid of, but I can't seem to manage that without also filtering out a lot of mitochondrial …
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Hello,
I tried to find the mitochondrial sequences in my assembly.
I assembled CLR PacBio reads using CANU for a mollusc genome.
As reference I used 2 mitochondrial genomes of the same species an…
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### Description of feature
The current stop-codon detection in ampliseq could be improved and supplemented with frame-shift detection by implementing [MACSE](https://doi.org/10.1093/molbev/msy159). T…
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Hi, I am trying your method to construct the mt genome, it is very fast which surprised me, My fastq data quality is not very good, I found the final result has multiple scaffolds and many same genes …
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Hello @kimrutherford and @ValWood, first congrats on managing to pass the submission pipeline!
I noticed that the new submission no longer includes the mitochondrial chromosome sequence. I know tha…
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Dear Changwei,
I was using autoMito to generate the 2 gfa files (raw and master) of the assembled Malus Domestica genome (as in Demo2) with the code as follows:
~/PMAT-1.5.3/bin/PMAT autoMito -i…