-
### Ask away!
Hi,
I think `pychopper` only trim and orient the full-length ONT-cDNA. Do we need trimming polyA tail before minimap2 alignment? I saw occassionally some misalign to genomic region.
-
Hi
Great work with trackplot
I currently plotted some reads which have polyA tail length as attached
is there a way to order these reads by either transcript ie same exon and intron and then by…
-
Hello developers, thank you for creating the Dorado tool. I have received a lot of help from it.
Recently I've been dealing with my nanopore DRS data, and at this stage we'd like to explore some poly…
x1han updated
2 weeks ago
-
Dear developers,
Recently I am trying some customized methods to complete the full-length measurement of the polya tail on nanopore.
However, I don’t know whether it is because the same primer is …
-
Hi,
I'm using cutadapt v4.9 installed with conda
Maybe my aim is a bit niche here, but I'd like to filter out any reads matching the polyA filter in cutadapt (rather than trimming the polyA whic…
-
common result from thinking about recursion in a language
-
Since you can export real number expressions from Coq, maybe it is possible to call [Polya](https://github.com/avigad/polya), too? @avigad or @rlewis1988 will know more about this.
-
It is clear that eventalign-dependent modules does not support R10.4, but it looks like polya uses non-eventaligned durations. Does this mean that polya should be fine to run on R10.4 data, or should …
-
Hi
I'm trying to replicate some splice junctions in my long-read Kinnex data. Out of a total of 115 splice junctions, I managed to replicate about 100 from quantification obtained via the isoseq/SQ…
-
Hi relipmoc,
I was wondering if you had a suggestion for doing contaminant sequence trimming from paired end reads that is not adapter trimming. An example that comes up frequently is trimming off th…
roryk updated
10 years ago