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Hi,
I've successfully used scvelo in the past on 10x data by using a similar pipeline as in the tutorial, i.e. by separately reading the (scanpy pre-processed) 10x data and the loom file generated …
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Current loading procedure:
- load mRNA from fasta
- load peptide from fasta, link to mRNA via regexp
Problems with this strategy:
- we don't know locations of genes on scaffolds
- we can't prop…
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**Is your feature request related to a problem? Please describe.**
From a discord discussion.
Mutations and execution of a Stage is always on the machine LibAFL runs at. For very slow targets it may…
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Some people might be more willing to try out splicing if they know it can only destroy one specific (small) channel. So it'd be nice to have a way to specify that in the config.
Sjors updated
11 months ago
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Hello, I am a student trying to analyse my RNAseq data for alternative splicing. I am trying to run rMATS and encountering many errors. Any pointers in this direction will be very useful. Thank you ve…
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Hi Yuanhua,
I want to perform differential splicing of different groups of single cells. Could you provide an example for groups as I did not find this in the manual.
Thanks
ghost updated
2 years ago
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Hello all, I have two questions about the complexity column in the .psi.gz output files I hope to seek help from you. 1) Are the values in this column a prediction of the complexity of the relevant no…
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Exception: no ID could be matched with cis- file; check your Darts-flat ID column or if you supplied the correct cis-feature file.
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Hi, Timbitz:
I'm using "Whippet" to compare alternative splicing of different samples from RNAseq data. The idea to identify AS events from event-level is really brilliant.
When I finished the Whipp…
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Since gff files have specific parent/child structure, they are a natural fit for representing splicing events as isoforms. Additionally, the gff files created by outrigger can be combined with the ori…