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UnicodeDecodeError: 'utf-8' codec can't decode byte 0xa7 in position 0: invalid start byte
When running with the repeat mask file, it always report the errors, and I do not know why.
$ velo…
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**Goal**
Development of a stand-alone fastq validation tool.
A flexible tool that can be applied to different single-cell technology types to check for the expected cell barcode.
It should also r…
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Hi, thanks so much for developing this great tool. I just run a 10x Genomics scRNA sequencing on a pool of hashtag multiplexed sample. I tried to use the cmd below to generate the cell-hashtag count m…
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I am doing 16S rna amplicon based ONT sequencing.
My target region is only of 1kb to 2kb in length.
Per sample reads is around 50K.
Will HEERO give me good error filtered estimates ?
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Dear Alex:
My name is Chen Weng, I'm a postdoc at Whitehead Institute. Thank you for the amazing tool!
We have some dataset that has very long "Cell barcode", up to 43bp. When I try to…
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Hello,
Thanks for making this tool. I have a quick question about the parameters involving cells.
User can input the number of cells expected in the run, and/or also input the list of cell barcod…
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Hello!
When I was using scTE, I encountered some problems and I wonder if I can get some advice.
### Question 1: Preprocessing BAM Files with Filtered Barcodes from CellRanger Output
I'm ex…
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I tried running through the vignette for the PBMC 1K dataset.
I was able to reproduce a list of 124,716 cell barcodes coming from the kallisto/bus pipeline.
However when I compared this list of …
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Hello,
Thanks for this package. I have a few quenstions about the data processing. For the droplet-based method, why we need to perform cellranger to obtain bam files and then use STARsolo? Can we…