-
Hi there, when using the SAM file generated from RUM, it was found that the tag for unique mapping (NH:i:1) is missing and there is no information on whether if the reads are uniquely aligned (except …
-
Hi,
I wonder if anyone in this community can help me figure out the reason behind the discrepancy in counts. Please see below:
I am using HTSeq-count to generate counts from two different types …
-
I'm getting a tool error, trying to apply some rules to a collection.
Rules
- Add column for identifier0.
- Add new column using I18-....-.. applied to column A
- Set column B as List I…
-
`checkCBUMI` may not work as expected in https://github.com/JiekaiLab/scTE/blob/d9a300ed4428fe395fe0f13fd86fb158b88dd528/scTE/base.py#L109
I find some record in bam that generated by CellRanger …
-
-
**Describe the bug**
tmp_DCC files didn't get combine to generate 4 output files i.e CircRNAcount, CircCoordinates, CircSkipJunctions and LinearCount
# Command line used for the command
circtoo…
-
```
What steps will reproduce the problem?
1. mapping 50 bp single end illumina reads to bacterial genome
2.
3.
What is the expected output? What do you see instead?
reported mapped # of reads does n…
-
Dear Alex,
I am posting the info from log files I got post running mapping with STAR. I am surprised by the difference in number of reads reported between ReadsPerGene.out.tab file and Log.final.ou…
-
```
If you set outSAMattributes as All then the sam file generated is incompatible
with Cufflinks. The error is something like no XS tag for a spliced read. But
when one uses outSAMattributes in st…
-
In order to take full advantage of MultiQC's aggregation report, it needs to find consistent sample names across various datasets. Thus, whenever possible, MultiQC should be using `element_identifier`…