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Hi @tjiangHIT ,
I am sorry, I encountered another problem. The attached bam file example has a clear heterozygous 97 base insertion at `1:1501010`.
![IGV_undetected](https://user-images.githubuser…
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I'd like to be able to do a conversion of a GAM output to BAM without realignment against the surjected path. I have a little script that converts regions of a GAM file to a coverage based view (attac…
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Hi, thanks for all the great work you are doing.
A quick question I could not find the answer to in the manual or google:
Does there exist a similar way in STAR to achive what bowtie does with t…
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Pretty self-explanatory. We're trying to eliminate the need to process data in fastq format, so it would be terrific if cutadapt could accept ubam input and write ubam output. (We probably don't need …
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Require:
- Description: Need to take a look at current apps. and define our strong points
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It would be nice if we could either give multiple output read lengths (one per file) or a read structure (similar to what exists in FastqToBam) in TrimFastq.
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Hello,
I want to try `imrep` with my RNA-seq data. I've aligned the `fastq` to genome with `STAR`, and saved the unmapped reads in `fastq` format (using `--outReadsUnmapped Fastx`). As you said, `S…
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Hi,
We have mapped our illumina short reads to our genome using typical commands with --SAM of chromap.
![45b8ab262cb63858d73611e96de4d93](https://user-images.githubusercontent.com/49976278/127…
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Hello I am trying to run the PASA training step (v. 1.8.11) with the following command but I get the following error
funannotate train -i $genome --cpus 1 -o $outdir -l $left -r $right --trinity $t…
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Checked on both samtools 1.9 on macOS and samtools 1.5 on Redhat Linux
Steps:
Decompress and index the attached bam file with `samtools index AV-WXa1591_m_scaffold1.bam`
then
`samtools mpileup …