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Hi team,
Have come back to Dorado after a little while:
`dorado basecaller dna_r10.4.1_e8.2_400bps_sup\@v4.1.0_5mCG_5hmCG\@v2/ benchmark.pod5 > calls.bam`
And get:
```
[2023-07-10 08:00:3…
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Hi Chris,
I'd like to harness the power of multiple GPUs for basecalling. Can you comment if this is possible/easily implementable?
I noticed @kishwarshafin seems to have this implemented in his for…
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Basecalling error rates should be based on alignment to reference genome.
LongReadSum currently calculates error rates based on the SAM MD tag.
See this line:
https://github.com/WGLab/LongReadSum…
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Hi,
We have just finished our first run of direct cDNA sequencing on our minion using the DCS109 library kit.
After basecalling with the guppy HAC we have tried running pychopper with the defaul…
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In a previous version of minknow (21.11.9, running in Ubuntu 18.04), the following configuration for read_filtering appeared in the sequencing config file /opt/ont/minknow/conf/package/sequencing/sequ…
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I read in your paper that Nanopolish performed the best among the 3 callers you tested. I have a few questions:
1. Nanopolish clusters neighboring CpGs when they are very close to each other. In th…
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Hi,
I used dna_r10.4.1_e8.2_400bps_sup@v4.2.0 in the following command :
`dorado duplex dna_r10.4.1_e8.2_400bps_sup@v4.2.0 pod5s/ > duplex.bam`
I got the folowing errors:
`[2023-09-29 12:46:04…
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Hi,
I am trying to do basecalling using Bonito on the nanopore-wgs-consortium NA12878 dataset. But I am getting the **can't locate attribute: 'sample_id' error**. Is there something I can do to make…
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### What happened?
Hello everyone!
I started epi2me lab's human variation workflow for the first time, and faced with dorado basecalling and clair3 problems. The error message was the following: …
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### What happened?
I am running the wf-human-variation pipeline on slurm.
The basecaller_cfg parameter has been set to: dna_r10.4.1_e8.2_400bps_hac@v4.0.0
I get a model not found error even though…