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# 生信爱好者周刊(第 110 期):
这里记录每周值得分享的生信相关内容,周日发布。
本杂志开源(GitHub: [openbiox/weekly](https://github.com/openbiox/weekly)),欢迎提交 issue,投稿或推荐生信相关内容。
[「生信周刊讨论区」](https://github.com/openbiox/weekly/discuss…
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Hi, I'm trying to basecall from pod5 files and keep running into the error below. I'm running Epi2me on a Linux machine. Would the pod5 folder being located on a mapped network drive be contributi…
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I'm going to process a bunch of sequencing datasets from museum samples, some stored under horrendous conditions and for decades. As a result, the DNA fragments are very short, often shorter than a re…
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Dear team:
Thanks for developing this tool which is really helpful.
I am trying to use it for a customer marker gene sets and I reformat the protein sequence and cds sequence as suggested here:
[#2…
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It seems dorado won't do basecall for polyA sequence either in the end of RNA or inside a plasmid construct. Is there a plan to include this feature soon?
yul96 updated
8 months ago
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When basecalling modified bases with `dorado` v0.5.0, using the modification models `5mC_5hmC` and `5mCG_5hmCG` stall basecalling randomly, i.e. sometimes `dorado` finishes basecalling, but mostly it …
janpb updated
9 months ago
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The remora provided 5hmC_5mC and 5mC models, but, how to distinguish 5mC and 5hmC base modification from the results? 'm' is single modified base code, what is the single modified base code for 5hmC?…
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Update the following URL to point to the GitHub repository of
the package you wish to submit to _Bioconductor_
- Repository: https://github.com/haibol2016/CleanUpRNAseq
Confirm the following by…
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Hi,
Thanks for this very good work. I was wondering if I can retrieve the embedding from DNA sequences that I would train and use them later then for down stream tasks. Can you please confirm that w…
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Hello,
I am facing a strange issue that I am not able to solve on my own and would appreciate any insight as to what I might be missing here. I have a dataset of reads from a plasmid spiked with o…