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Hi,
I was just processing a bunch of reads with dorado trim (0.7.3) and noticed that it keeps saying:
`[info] > Simplex reads basecalled: 456331`
And if I turn verbose on:
```
[debug] Total…
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Dear developpers
As already discussed in [issue 621](https://github.com/nanoporetech/dorado/issues/621), a progress bar only visible interactively (outside a log file), is not very useful when you …
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Hi,
Is it possible or would it be possible for dorado to handle a sample sheet across multiple positions/flow cells, essentially allowing the same sample or barcoded sample to be loaded across multip…
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Hello. I was reviewing some of my recent RNA004 direct RNA seq data to see what modifications I could call from them. I have six replicates here, and I called m6A, m5C, and pseudouridine for all of th…
mbosm updated
4 weeks ago
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I use the info-file quite often, either with awk one liners or importing the file in R. I have been using them with Illumina data, with great success. Now I am trying to use them with Nanopore data, w…
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Would be nice if you could join up `rftools` with dorado with pipes. Something like:
```console
dorado basecaller hac pod5s/ | rftools split-bam --bam-file - --unblocked-read-ids ...
```
Not s…
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Hi,
I am trying to assemble the genome of a worm, and I was hoping that minipolish would detect the mitochondrial genome and output it in the gfa file.
I have sequencing one of my worm species a l…
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Hi devlopers of Dorado,
First, thank you for providing this great software.
I noticed that after issue #532 , `RG` tag and some other tag is automatically added to the FASTQ header, which I …
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Hi,
It will be great if the `--emit-summary` option for `dorado demux` also exists with `dorado basecaller`. With this new feature, it will be possible to perform basecalling, demultiplexing and cr…
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When basecalling RNA2 data with dorado 0.5.3 it performs automatic read splitting via an open pore detection algorithm.
This algorithm is broken, as it not only detects actual open pores, but also…