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I tried adVNTR on PacBio data and the output bed file has only None values in R1 and R2 columns. It basically does not genotype any tandem repeat region.
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Lets decide how we go with this.
I think it makes sense to test current assembly with real data that needs analysis to gauge status.
I sugget two ways forward -
More PacBio or Nanopore....
sr320 updated
6 years ago
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[Pilon](https://github.com/broadinstitute/pilon) is a good example of how to combine PacBio and Illumina data. Add a section on using Pilon.
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How do I use BString / BStringSet for values > 127?
PacBio data uses 0:255 ranges for fp/rp/fi/ri tags. I am trying to use BioStrings to work with these tags.
But I ran into trouble, for values …
gevro updated
5 months ago
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I'm trying to run some nanopore test reads through mothur but am getting stuck at the very beginning. I was going to follow your pacbio example so was trying to start with fastq.info. My data are conc…
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Hello,
I have implemented RNA-Bloom on a subset of the PacBio bulk transcriptomic data and achieved the expected results. However, I noticed that some steps in RNA-Bloom take a considerable amount …
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Hi,
I installed circlator from here (github) and after installing all the dependencies listed here I ran 'circlator progcheck' but this is the error I keep getting and I have tried a lot of things i…
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Hi @benjjneb this isn't an issue with dada2 but more a general question. We're using a slightly modified version of the big data workflow implemented in Nextflow for processing PacBio data, but the k…
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Hi Wouter,
I'm trying to compare sequencing data from two different sequencing platforms (Pacbio sequel and revio) on the same graph but I can only get a graph where both have the same surface area…
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I have a question about internal timing of masurca. Does masurca trim adaptors from long read sequencing data set (ONT/PacBio)?