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Dear Velocyto Team,
Our lab used a modified Smart-seq2 protocol to allow for multiplexed single-cell RNA-seq. Primers of RT reaction were designed with cell-specific barcodes and unique molecular ide…
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Thank you for this powerful tool! I ran this on my own 10x data. I encountered an error when running the Preprocess_Gene_module.py.
![图片](https://github.com/cmzuo11/stKeep/assets/166676441/66b11cbc…
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We would like the simulation function vary the following parameters. Next to each parameter is a link to relevant existing code:
* [x] Number of samples in each group
* [x] Number of genes
* [x] …
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Hello!
I'm having a slight issue with the annotations generated by EGAPx. When I translate the CDS into a protein sequence, I'm seeing a lot of premature stop codons. This is a species where I am s…
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Thanks @clairemcleod for the RNA sequencing introduction in #13! There was interest at the meetup yesterday (slides are [here](https://slides.com/dhimmel/cognomathon/)) to provide a list of resources …
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Talking through strain representations at the data-representation scrum today, we identified an issue. I'll do my best to summarize with an example:
1) Strains are designed and sequences/annotations …
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One of the fastq files I'm processing was carried out using the NEXTflex™ Small RNA-Seq Kit for library prep which uses an adapter sequence with Ns in it
NNNNTGGAATTCTCGGGTGCCAAGG
I tried passi…
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hello again..!
i run the deeplexicon but error is occured...
how can i solve this..?
![image](https://github.com/user-attachments/assets/858dca84-3314-4b5f-ab48-b9f00b905853)
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Hi,
First, apologies if this is not the best place to ask this question. I'm doing gene annotation for a non-model rodent, and I have tried several approaches:
1. BRAKER2: with a large protein dat…